Chapter 8: Animal Cell Culture — Online MCQ Test
BIOTECHNOLOGY · Grade 12 · CBSE(NCERT)
Practice Chapter 8: Animal Cell Culture with a free chapter-wise online MCQ test.
This chapter covers: Animal Cell Culture Primary Culture Cell Line In Vitro Culture Growth Medium Monoclonal Antibodies Vaccine Production Cell Maintenance Tissue Culture.
AI-generated questions from basic to board-exam level, with instant results and explanations.
Chapter 8: Animal Cell Culture — Important Questions & Answers
Animal cell culture is best described as the growth of animal cells:
- A. Outside the organism under controlled conditions
- B. Only inside a living animal
- C. Only in soil-based nutrient medium
- D. Without any nutrient requirement
Answer: A. Outside the organism under controlled conditions
Animal cell culture involves maintaining and growing animal cells in vitro, i.e., outside the body under controlled laboratory conditions.
Animal cell culture involves maintaining and growing animal cells in vitro, i.e., outside the body under controlled laboratory conditions.
A culture started directly from cells, tissues, or organs taken from an animal is called:
- A. Primary culture
- B. Continuous cell line
- C. Hybridoma culture
- D. Cloned culture
Answer: A. Primary culture
Primary culture is established directly from animal tissue and contains cells that have not yet been repeatedly subcultured.
Primary culture is established directly from animal tissue and contains cells that have not yet been repeatedly subcultured.
Which of the following is commonly added to animal cell culture medium as a source of growth factors?
- A. Fetal bovine serum
- B. Agar
- C. Cellulose
- D. Starch
Answer: A. Fetal bovine serum
Fetal bovine serum supplies growth factors, hormones, attachment factors, and proteins required by many animal cells.
Fetal bovine serum supplies growth factors, hormones, attachment factors, and proteins required by many animal cells.
In many animal cell cultures, a CO2 incubator is used mainly to:
- A. Help maintain the pH of bicarbonate-buffered medium
- B. Kill all bacteria in the culture
- C. Freeze the cells rapidly
- D. Provide nitrogen for protein synthesis
Answer: A. Help maintain the pH of bicarbonate-buffered medium
A controlled CO2 atmosphere helps maintain pH when bicarbonate buffer is present in the medium.
A controlled CO2 atmosphere helps maintain pH when bicarbonate buffer is present in the medium.
Which sequence is most appropriate for establishing a primary animal cell culture from a tissue sample?
- A. Collect tissue aseptically → Disaggregate cells → Seed in suitable medium
- B. Freeze tissue → Add agar → Expose to open air
- C. Boil tissue → Add antibiotics → Incubate without medium
- D. Dry tissue → Grind with soil → Store at room temperature
Answer: A. Collect tissue aseptically → Disaggregate cells → Seed in suitable medium
Primary culture requires aseptic collection, mechanical or enzymatic disaggregation, and inoculation into an appropriate culture medium.
Primary culture requires aseptic collection, mechanical or enzymatic disaggregation, and inoculation into an appropriate culture medium.
Which observation most strongly suggests microbial contamination in an animal cell culture?
- A. Sudden turbidity and rapid pH change in the medium
- B. Cells forming a uniform monolayer
- C. Slow but steady increase in cell number
- D. Cells excluding trypan blue dye
Answer: A. Sudden turbidity and rapid pH change in the medium
Microbial contamination often causes turbidity, unexpected pH change, and unhealthy cell morphology.
Microbial contamination often causes turbidity, unexpected pH change, and unhealthy cell morphology.
A researcher has cells from a freshly isolated tissue that have not yet been passaged. After several subcultures, they are referred to as a cell line. Which statement is correct?
- A. The initial culture is primary culture; repeated subculturing can give rise to a cell line
- B. The initial culture is a continuous cell line; subculture makes it primary
- C. A primary culture and a cell line are always identical terms
- D. Subculturing always kills all animal cells
Answer: A. The initial culture is primary culture; repeated subculturing can give rise to a cell line
Primary culture is derived directly from tissue, while a cell line is obtained after the cells are subcultured or passaged.
Primary culture is derived directly from tissue, while a cell line is obtained after the cells are subcultured or passaged.
A confluent culture flask contains 4 × 10^6 viable cells. If cells are divided equally into four new flasks, what is the split ratio and cells per flask?
- A. 1:4 split; 1 × 10^6 cells per flask
- B. 4:1 split; 4 × 10^6 cells per flask
- C. 1:2 split; 2 × 10^6 cells per flask
- D. 1:8 split; 0.25 × 10^6 cells per flask
Answer: A. 1:4 split; 1 × 10^6 cells per flask
Dividing one flask into four new flasks is a 1:4 split, and 4 × 10^6 cells divided by 4 gives 1 × 10^6 cells per flask.
Dividing one flask into four new flasks is a 1:4 split, and 4 × 10^6 cells divided by 4 gives 1 × 10^6 cells per flask.
In HAT selection during hybridoma production, which combination allows hybridoma cells to survive?
- A. Immortality from myeloma cells and salvage pathway enzyme from B cells
- B. Antibody production from myeloma cells and absence of nuclei from B cells
- C. Cell wall formation from B cells and plasmids from myeloma cells
- D. Agar digestion from myeloma cells and chlorophyll from B cells
Answer: A. Immortality from myeloma cells and salvage pathway enzyme from B cells
In HAT medium, aminopterin blocks de novo nucleotide synthesis; hybridomas survive because they inherit immortality from myeloma cells and salvage pathway ability from B cells.
In HAT medium, aminopterin blocks de novo nucleotide synthesis; hybridomas survive because they inherit immortality from myeloma cells and salvage pathway ability from B cells.
Which statement is most accurate about contact inhibition in animal cell culture?
- A. Normal adherent cells often stop dividing when they touch neighbouring cells, while transformed continuous cells may lose this control
- B. Contact inhibition means cells divide only after complete overcrowding
- C. Contact inhibition is a property found only in bacterial colonies
- D. Contact inhibition is caused by freezing cells in liquid nitrogen
Answer: A. Normal adherent cells often stop dividing when they touch neighbouring cells, while transformed continuous cells may lose this control
Many normal animal cells show contact inhibition and stop growing at confluence; transformed or continuous cell lines may lose this regulation.
Many normal animal cells show contact inhibition and stop growing at confluence; transformed or continuous cell lines may lose this regulation.