Chapter 1: An Overview of Recombinant DNA Technology — Online MCQ Test
BIOTECHNOLOGY · Grade 12 · CBSE(NCERT)
Practice Chapter 1: An Overview of Recombinant DNA Technology with a free chapter-wise online MCQ test.
This chapter covers: Recombinant DNA Genetic Engineering DNA Isolation DNA Modification Gene Splicing Recombinant Molecules Human Insulin Therapeutic Proteins Restriction Enzymes Biotechnology.
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Chapter 1: An Overview of Recombinant DNA Technology — Important Questions & Answers
Recombinant DNA technology mainly involves the formation of DNA molecules by joining DNA from:
- A. Two different sources
- B. Only identical cells
- C. Only RNA viruses
- D. Only mitochondria
Answer: A. Two different sources
Recombinant DNA is formed by joining DNA fragments from two different sources, often from different organisms.
Recombinant DNA is formed by joining DNA fragments from two different sources, often from different organisms.
Which enzyme is commonly called molecular scissors in recombinant DNA technology?
- A. DNA ligase
- B. Restriction endonuclease
- C. DNA polymerase
- D. Reverse transcriptase
Answer: B. Restriction endonuclease
Restriction endonucleases cut DNA at specific recognition sequences and are therefore called molecular scissors.
Restriction endonucleases cut DNA at specific recognition sequences and are therefore called molecular scissors.
A student wants to clone a gene in a plasmid. Which sequence of steps is most appropriate?
- A. Cut vector and insert, ligate them, introduce into host cells
- B. Introduce vector into host, cut host chromosome, ligate proteins
- C. Translate gene, cut protein, insert into plasmid
- D. Ligate intact cells, cut plasmid, isolate amino acids
Answer: A. Cut vector and insert, ligate them, introduce into host cells
In cloning, the gene insert and vector are cut with suitable restriction enzymes, joined using ligase, and introduced into host cells.
In cloning, the gene insert and vector are cut with suitable restriction enzymes, joined using ligase, and introduced into host cells.
A researcher ligates a human gene into the middle of an antibiotic resistance gene of a plasmid. What is the most likely effect on that resistance gene?
- A. It becomes insertionally inactivated
- B. It becomes a restriction enzyme
- C. It duplicates the bacterial chromosome
- D. It converts into mRNA permanently
Answer: A. It becomes insertionally inactivated
Insertion of foreign DNA within a functional marker gene can disrupt it, a principle called insertional inactivation.
Insertion of foreign DNA within a functional marker gene can disrupt it, a principle called insertional inactivation.
A DNA sequence reads 5'-GAATTC-3' on one strand and 3'-CTTAAG-5' on the other. Why is it important for EcoRI?
- A. It is a palindromic recognition sequence cut by EcoRI
- B. It is a codon sequence for insulin only
- C. It is the bacterial origin of replication
- D. It is a protein sequence recognized by ligase
Answer: A. It is a palindromic recognition sequence cut by EcoRI
EcoRI recognizes the palindromic sequence GAATTC and cuts it to produce sticky ends.
EcoRI recognizes the palindromic sequence GAATTC and cuts it to produce sticky ends.