Chapter 10: Bioprocessing and Biomanufacturing — Online MCQ Test
BIOTECHNOLOGY · Grade 12 · CBSE(NCERT)
Practice Chapter 10: Bioprocessing and Biomanufacturing with a free chapter-wise online MCQ test.
This chapter covers: Bioreactor Fermenter Bioprocessing Biomanufacturing Downstream Processing Fermentation pH Temperature Scale Up Product Purification Industrial Biotechnology.
AI-generated questions from basic to board-exam level, with instant results and explanations.
Chapter 10: Bioprocessing and Biomanufacturing — Important Questions & Answers
In industrial biotechnology, a bioreactor is mainly used to provide a controlled environment for:
- A. Growth of cells or enzymes to produce a desired product
- B. Storage of purified proteins at room temperature
- C. Packaging of finished pharmaceutical products
- D. Microscopic observation of stained cells
Answer: A. Growth of cells or enzymes to produce a desired product
A bioreactor provides controlled conditions such as temperature, pH, aeration and agitation for biological production processes.
A bioreactor provides controlled conditions such as temperature, pH, aeration and agitation for biological production processes.
The process of separating and purifying a product after fermentation is called:
- A. Upstream processing
- B. Downstream processing
- C. Inoculum development
- D. Media sterilization
Answer: B. Downstream processing
Downstream processing includes recovery, separation, purification and formulation of the desired product after the bioprocess.
Downstream processing includes recovery, separation, purification and formulation of the desired product after the bioprocess.
Which statement best describes upstream processing in biomanufacturing?
- A. It includes product crystallization and packaging only
- B. It includes strain selection, media preparation, sterilization and fermentation
- C. It refers only to disposal of solid waste from the plant
- D. It begins only after the product is purified
Answer: B. It includes strain selection, media preparation, sterilization and fermentation
Upstream processing involves all steps needed to grow the production organism and produce the product before recovery begins.
Upstream processing involves all steps needed to grow the production organism and produce the product before recovery begins.
During scale-up from laboratory flask to industrial fermenter, which factor is most likely to become difficult to maintain uniformly?
- A. Oxygen transfer and mixing throughout the larger volume
- B. The chemical formula of water
- C. The genetic code of the organism
- D. The colour of the fermenter vessel
Answer: A. Oxygen transfer and mixing throughout the larger volume
As volume increases, mixing, heat removal and oxygen transfer become more challenging, affecting culture performance.
As volume increases, mixing, heat removal and oxygen transfer become more challenging, affecting culture performance.
While scaling up an aerobic microbial process, the lab yield is not reproduced at pilot scale even though the same medium and strain are used. Which explanation is most scientifically valid?
- A. Large reactors may have gradients of oxygen, nutrients, pH and temperature that are absent or smaller in flasks
- B. The organism cannot grow in any vessel larger than a flask
- C. Scale-up changes the genetic code of all microbes automatically
- D. Downstream processing prevents fermentation from occurring
Answer: A. Large reactors may have gradients of oxygen, nutrients, pH and temperature that are absent or smaller in flasks
Scale-up changes physical conditions such as mixing, mass transfer and heat transfer, which can alter growth and product formation.
Scale-up changes physical conditions such as mixing, mass transfer and heat transfer, which can alter growth and product formation.