Chapter 4: Applications of Recombinant DNA Technology — Online MCQ Test
BIOTECHNOLOGY · Grade 12 · CBSE(NCERT)
Practice Chapter 4: Applications of Recombinant DNA Technology with a free chapter-wise online MCQ test.
This chapter covers: Genetically Modified Organisms GMO Gene Therapy Recombinant Proteins Human Insulin Vaccines Industrial Enzymes Agricultural Biotechnology Medical Biotechnology Industrial Biotechno....
AI-generated questions from basic to board-exam level, with instant results and explanations.
Chapter 4: Applications of Recombinant DNA Technology — Important Questions & Answers
A genetically modified organism (GMO) is best defined as an organism whose genetic material has been:
- A. Altered using recombinant DNA technology
- B. Changed only by natural selection
- C. Modified only by conventional breeding
- D. Destroyed using restriction enzymes
Answer: A. Altered using recombinant DNA technology
A GMO contains DNA that has been deliberately altered using genetic engineering or recombinant DNA techniques.
A GMO contains DNA that has been deliberately altered using genetic engineering or recombinant DNA techniques.
The first recombinant DNA-derived human protein approved for therapeutic use was:
- A. Human insulin
- B. Human growth hormone
- C. Interferon-alpha
- D. Erythropoietin
Answer: A. Human insulin
Recombinant human insulin was among the earliest and most important recombinant proteins used in medicine.
Recombinant human insulin was among the earliest and most important recombinant proteins used in medicine.
A farmer grows Bt cotton and observes reduced damage by bollworms. The protection is mainly due to:
- A. Production of an insecticidal protein in plant tissues
- B. Increased production of cellulose in cotton fibres
- C. Presence of antibiotic resistance genes in pollen
- D. Faster photosynthesis in cotton leaves
Answer: A. Production of an insecticidal protein in plant tissues
Bt cotton expresses Cry proteins that are toxic to specific insect larvae such as bollworms.
Bt cotton expresses Cry proteins that are toxic to specific insect larvae such as bollworms.
The insecticidal Bt protein is usually harmless to humans but toxic to specific insects because:
- A. It is activated under alkaline conditions in the insect gut and binds to specific gut receptors
- B. It blocks photosynthesis in insect cells
- C. It destroys insect DNA in the soil before feeding
- D. It acts as a universal poison for all animal cells
Answer: A. It is activated under alkaline conditions in the insect gut and binds to specific gut receptors
Cry protoxins become active in the alkaline gut of susceptible insects and bind to specific receptors, causing cell lysis.
Cry protoxins become active in the alkaline gut of susceptible insects and bind to specific receptors, causing cell lysis.
In recombinant insulin production, why was it useful to produce A and B chains separately rather than extracting insulin from animal pancreas?
- A. It allowed production of human insulin chains that could be joined to form active insulin without animal-source contaminants
- B. It prevented the need for disulfide bond formation in insulin
- C. It made insulin a DNA molecule instead of a protein
- D. It produced insulin that acts only in bacteria and not in humans
Answer: A. It allowed production of human insulin chains that could be joined to form active insulin without animal-source contaminants
Separate recombinant production of A and B chains enabled formation of human insulin with reduced dependence on animal pancreas extracts.
Separate recombinant production of A and B chains enabled formation of human insulin with reduced dependence on animal pancreas extracts.