Unit 9: Biotechnology and Its Applications — Online MCQ Test
BIOLOGY · NEET (All) · NEET
Practice Unit 9: Biotechnology and Its Applications with a free chapter-wise online MCQ test.
This chapter covers: Principles and process of Biotechnology: Genetic engineering (Recombinant DNA technology). Application of Biotechnology in health and agriculture: Human insulin and vaccine product....
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Unit 9: Biotechnology and Its Applications — Important Questions & Answers
Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to :
- A. Fungal diseases
- B. Plant nematodes
- C. Insect predators
- D. Insect pests
Answer: D. Insect pests
Bt cotton is genetically modified to produce insecticidal proteins (Cry proteins) that are toxic to specific insect pests (e.g., bollworms).
Bt cotton is genetically modified to produce insecticidal proteins (Cry proteins) that are toxic to specific insect pests (e.g., bollworms).
Identify the wrong statement with regard to Restriction Enzymes.
- A. They cut the strand of DNA at palindromic sites.
- B. They are useful in genetic engineering.
- C. Sticky ends can be joined by using DNA ligases.
- D. Each restriction enzyme functions by inspecting the length of a DNA sequence.
Answer: D. Each restriction enzyme functions by inspecting the length of a DNA sequence.
Restriction enzymes do not inspect the length; they inspect the specific nucleotide sequence (recognition site) to cut DNA.
Restriction enzymes do not inspect the length; they inspect the specific nucleotide sequence (recognition site) to cut DNA.
Match the following columns and select the correct option: (a) Bt cotton, (b) Adenosine deaminase deficiency, (c) RNAi, (d) PCR.
- A. 1) (iii) (i) (iv) (ii) (Error in text mapping)
- B. 2) (ii) (iii) (iv) (i) (Error in text mapping)
- C. 3) (i) (ii) (iii) (iv) (Error in text mapping)
- D. 4) (iv) (i) (ii) (iii)
Answer: D. 4) (iv) (i) (ii) (iii)
Bt cotton uses Bacillus thuringiensis (a-iv). Adenosine deaminase deficiency is treated by gene therapy (b-i). RNAi is for cellular defense (c-ii). PCR is used for detection of HIV (d-iii).
Bt cotton uses Bacillus thuringiensis (a-iv). Adenosine deaminase deficiency is treated by gene therapy (b-i). RNAi is for cellular defense (c-ii). PCR is used for detection of HIV (d-iii).
The specific palindromic sequence which is recognized by EcoRI is:
- A. 5' - GGAACC - 3' / 3'- CCTTGG-5'
- B. 5' - CTTAAG - 3' / 3' - GAATTC - 5'
- C. 5' - GGATCC - 3' / 3' - CCTAGG - 5'
- D. 5' - GAATTC - 3' / 3' - CTTAAG - 5'
Answer: D. 5' - GAATTC - 3' / 3' - CTTAAG - 5'
EcoRI recognizes the palindromic sequence 5'-GAATTC-3', which is cleaved between G and A.
EcoRI recognizes the palindromic sequence 5'-GAATTC-3', which is cleaved between G and A.
In gel electrophoresis, separated DNA fragments can be visualized with the help of:
- A. Ethidium bromide in UV radiation
- B. Acetocarmine in UV radiation
- C. Ethidium bromide in infrared radiation
- D. Acetocarmine in bright blue light
Answer: A. Ethidium bromide in UV radiation
Ethidium bromide is an intercalating dye that fluoresces orange under UV light, used to detect DNA in agarose gels.
Ethidium bromide is an intercalating dye that fluoresces orange under UV light, used to detect DNA in agarose gels.
The sequence that controls the copy number of the linked DNA in the vector, is termed:
- A. Ori site
- B. Palindromic sequence
- C. Recognition site
- D. Selectable marker
Answer: A. Ori site
The Origin of replication (Ori) site is the sequence where replication starts and it controls the copy number of the DNA insert.
The Origin of replication (Ori) site is the sequence where replication starts and it controls the copy number of the DNA insert.
Polymerase chain reaction (PCR) amplifies DNA following the equation.
- A. N2
- B. 2^n
- C. 2n + 1
- D. 2N2
Answer: B. 2^n
PCR amplification follows exponential growth represented by 2 to the power of n cycles.
PCR amplification follows exponential growth represented by 2 to the power of n cycles.
In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
- A. Using ampicillin & tetracyclin containing medium plate.
- B. Blue color colonies will be selected.
- C. White color colonies will be selected.
- D. Blue color colonies grown on ampicillin plates can be selected.
Answer: C. White color colonies will be selected.
Insertion of foreign DNA at a site within the lacZ gene (blue-white screening) results in insertional inactivation of the gene, causing colonies to remain white instead of blue.
Insertion of foreign DNA at a site within the lacZ gene (blue-white screening) results in insertional inactivation of the gene, causing colonies to remain white instead of blue.
The taq polymerase enzyme is obtained from:
- A. Thermus aquaticus
- B. Thiobacillus ferroxidans
- C. Bacillus subtilis
- D. Pseudomonas putida
Answer: A. Thermus aquaticus
Taq polymerase is a thermostable DNA polymerase isolated from the thermophilic bacterium Thermus aquaticus.
Taq polymerase is a thermostable DNA polymerase isolated from the thermophilic bacterium Thermus aquaticus.
Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called
- A. Bio-infringement
- B. Bioexploitation
- C. Biodegradation
- D. Biopiracy
Answer: D. Biopiracy
Biopiracy refers to the use of bioresources by companies without proper authorization or compensatory payment to the concerned countries and their people.
Biopiracy refers to the use of bioresources by companies without proper authorization or compensatory payment to the concerned countries and their people.