Chapter 2: Microscopy — Online MCQ Test
MICROBIOLOGY · CLASS 11th · Tamil Nadu State Board
Practice Chapter 2: Microscopy with a free chapter-wise online MCQ test.
This chapter covers: Focusing on visualization tools this chapter covers light microscopy dark-field phase-contrast fluorescence and electron microscopy TEM and SEM. Students study magnification princi....
AI-generated questions from basic to board-exam level, with instant results and explanations.
Chapter 2: Microscopy — Important Questions & Answers
Which microscope uses visible light and lenses to magnify small objects?
- A. Electron microscope
- B. Light microscope
- C. Scanning electron microscope
- D. Fluorescence microscope
Answer: B. Light microscope
A light microscope uses visible light and glass lenses for magnification. It is the most basic and commonly used microscope in biology.
A light microscope uses visible light and glass lenses for magnification. It is the most basic and commonly used microscope in biology.
The ability of a microscope to distinguish two closely placed objects as separate is called:
- A. Magnification
- B. Resolution
- C. Contrast
- D. Reflection
Answer: B. Resolution
Resolution refers to the resolving power of a microscope. It determines how clearly two nearby points can be seen as separate.
Resolution refers to the resolving power of a microscope. It determines how clearly two nearby points can be seen as separate.
A student wants to see bacteria with increased contrast against a dark background. Which microscope should be used?
- A. Dark-field microscope
- B. Compound microscope
- C. Stereo microscope
- D. Polarizing microscope
Answer: A. Dark-field microscope
Dark-field microscopy makes the specimen appear bright on a dark background. It is useful for viewing very small, unstained organisms.
Dark-field microscopy makes the specimen appear bright on a dark background. It is useful for viewing very small, unstained organisms.
Which statement best compares SEM and TEM?
- A. SEM shows internal structure, TEM shows surface topography
- B. Both use visible light and glass lenses
- C. SEM shows surface details, TEM shows internal structure
- D. Both are used only for living cells
Answer: C. SEM shows surface details, TEM shows internal structure
Scanning electron microscopy provides a three-dimensional view of the surface. Transmission electron microscopy reveals internal structures of thin specimens.
Scanning electron microscopy provides a three-dimensional view of the surface. Transmission electron microscopy reveals internal structures of thin specimens.
Which statement about numerical aperture is correct?
- A. It is the total magnification of the microscope
- B. It represents the light-gathering ability of the lens
- C. It is the distance between objective and eyepiece
- D. It is the thickness of the specimen
Answer: B. It represents the light-gathering ability of the lens
Numerical aperture indicates how much light the lens can collect and resolve. A higher NA improves image detail and resolution.
Numerical aperture indicates how much light the lens can collect and resolve. A higher NA improves image detail and resolution.