Chapter Exam

Chapter 2: Microscopy — Online MCQ Test

Q: Which microscope uses visible light and lenses to magnify small objects?

Answer: B. Light microscope — A light microscope uses visible light and glass lenses for magnification. It is the most basic and commonly used microscope in biology.

Q: The ability of a microscope to distinguish two closely placed objects as separate is called:

Answer: B. Resolution — Resolution refers to the resolving power of a microscope. It determines how clearly two nearby points can be seen as separate.

Q: A student wants to see bacteria with increased contrast against a dark background. Which microscope should be used?

Answer: A. Dark-field microscope — Dark-field microscopy makes the specimen appear bright on a dark background. It is useful for viewing very small, unstained organisms.

MICROBIOLOGY · CLASS 11th · Tamil Nadu State Board

Practice Chapter 2: Microscopy with a free chapter-wise online MCQ test for Tamil Nadu State Board CLASS 11th MICROBIOLOGY. This chapter covers: Focusing on visualization tools this chapter covers light microscopy dark-field phase-contrast fluorescence and electron microscopy TEM and SEM. Students study magnification princi.... AI-generated questions from basic to board-exam level, with instant results and explanations.

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Chapter 2: Microscopy — Important Questions & Answers (FAQ)

Frequently asked questions from Tamil Nadu State Board CLASS 11th MICROBIOLOGY — Chapter 2: Microscopy, with answers and explanations. These are sample questions; the exam has its own separate question set.

Which microscope uses visible light and lenses to magnify small objects?

A. Electron microscope
B. Light microscope ✓
C. Scanning electron microscope
D. Fluorescence microscope

Answer: B. Light microscope
A light microscope uses visible light and glass lenses for magnification. It is the most basic and commonly used microscope in biology.

The ability of a microscope to distinguish two closely placed objects as separate is called:

A. Magnification
B. Resolution ✓
C. Contrast
D. Reflection

Answer: B. Resolution
Resolution refers to the resolving power of a microscope. It determines how clearly two nearby points can be seen as separate.

A student wants to see bacteria with increased contrast against a dark background. Which microscope should be used?

A. Dark-field microscope ✓
B. Compound microscope
C. Stereo microscope
D. Polarizing microscope

Answer: A. Dark-field microscope
Dark-field microscopy makes the specimen appear bright on a dark background. It is useful for viewing very small, unstained organisms.

Which statement best compares SEM and TEM?

A. SEM shows internal structure, TEM shows surface topography
B. Both use visible light and glass lenses
C. SEM shows surface details, TEM shows internal structure ✓
D. Both are used only for living cells

Answer: C. SEM shows surface details, TEM shows internal structure
Scanning electron microscopy provides a three-dimensional view of the surface. Transmission electron microscopy reveals internal structures of thin specimens.

Which statement about numerical aperture is correct?

A. It is the total magnification of the microscope
B. It represents the light-gathering ability of the lens ✓
C. It is the distance between objective and eyepiece
D. It is the thickness of the specimen

Answer: B. It represents the light-gathering ability of the lens
Numerical aperture indicates how much light the lens can collect and resolve. A higher NA improves image detail and resolution.

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