Chapter Exam

Chapter 5: Cultivation of Microorganisms — Online MCQ Test

Q: Which culture medium contains a known chemical composition and exact quantity of ingredients?

Answer: B. Synthetic medium — Synthetic media have a precisely known chemical composition. They are prepared from pure chemicals in exact amounts.

Q: A pure culture is a culture that contains:

Answer: A. Only one species of microorganism — A pure culture has only one species or one kind of microorganism. It is essential for studying characteristics of a single microbe.

Q: Which method is most suitable for obtaining isolated colonies from a mixed culture when the number of viable cells must also be estimated?

Answer: B. Pour plate method — The pour plate method can be used to count viable cells and also produce isolated colonies. It is useful when both isolation and enumeration are needed.

MICROBIOLOGY · CLASS 11th · Tamil Nadu State Board

Practice Chapter 5: Cultivation of Microorganisms with a free chapter-wise online MCQ test for Tamil Nadu State Board CLASS 11th MICROBIOLOGY. This chapter covers: This chapter details culture media types synthetic complex selective differential and pure culture isolation methods. Students study streak plate pour plate slant cultures and anae.... AI-generated questions from basic to board-exam level, with instant results and explanations.

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Chapter 5: Cultivation of Microorganisms — Important Questions & Answers (FAQ)

Frequently asked questions from Tamil Nadu State Board CLASS 11th MICROBIOLOGY — Chapter 5: Cultivation of Microorganisms, with answers and explanations. These are sample questions; the exam has its own separate question set.

Which culture medium contains a known chemical composition and exact quantity of ingredients?

A. Complex medium
B. Synthetic medium ✓
C. Differential medium
D. Enriched medium

Answer: B. Synthetic medium
Synthetic media have a precisely known chemical composition. They are prepared from pure chemicals in exact amounts.

A pure culture is a culture that contains:

A. Only one species of microorganism ✓
B. Only one type of nutrient
C. Only living cells and no dead cells
D. Microorganisms from different habitats

Answer: A. Only one species of microorganism
A pure culture has only one species or one kind of microorganism. It is essential for studying characteristics of a single microbe.

Which of the following is a characteristic of complex media?

A. Exact chemical composition is known
B. Contains ingredients of unknown precise composition ✓
C. Used only for anaerobes
D. Always selective

Answer: B. Contains ingredients of unknown precise composition
Complex media contain peptone, yeast extract, or other ingredients whose exact chemical composition is not fully known. They support the growth of many microorganisms.

Which method is most suitable for obtaining isolated colonies from a mixed culture when the number of viable cells must also be estimated?

A. Streak plate method
B. Pour plate method ✓
C. Slant culture method
D. Broth culture method

Answer: B. Pour plate method
The pour plate method can be used to count viable cells and also produce isolated colonies. It is useful when both isolation and enumeration are needed.

Which pairing is correctly matched?

A. Streak plate — estimation of viable count
B. Pour plate — isolation of colonies and viable count ✓
C. Slant culture — only anaerobic cultivation
D. Anaerobic jar — growth of aerobic bacteria with extra oxygen

Answer: B. Pour plate — isolation of colonies and viable count
Pour plate is used for both colony isolation and viable count estimation. The other pairs are incorrectly matched with their main uses.

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Chapter 6: Microbial Nutrition and Growth