Chapter 5: Cultivation of Microorganisms — Online MCQ Test
MICROBIOLOGY · CLASS 11th · Tamil Nadu State Board
Practice Chapter 5: Cultivation of Microorganisms with a free chapter-wise online MCQ test.
This chapter covers: This chapter details culture media types synthetic complex selective differential and pure culture isolation methods. Students study streak plate pour plate slant cultures and anae....
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Chapter 5: Cultivation of Microorganisms — Important Questions & Answers
Which culture medium contains a known chemical composition and exact quantity of ingredients?
- A. Complex medium
- B. Synthetic medium
- C. Differential medium
- D. Enriched medium
Answer: B. Synthetic medium
Synthetic media have a precisely known chemical composition. They are prepared from pure chemicals in exact amounts.
Synthetic media have a precisely known chemical composition. They are prepared from pure chemicals in exact amounts.
A pure culture is a culture that contains:
- A. Only one species of microorganism
- B. Only one type of nutrient
- C. Only living cells and no dead cells
- D. Microorganisms from different habitats
Answer: A. Only one species of microorganism
A pure culture has only one species or one kind of microorganism. It is essential for studying characteristics of a single microbe.
A pure culture has only one species or one kind of microorganism. It is essential for studying characteristics of a single microbe.
Which of the following is a characteristic of complex media?
- A. Exact chemical composition is known
- B. Contains ingredients of unknown precise composition
- C. Used only for anaerobes
- D. Always selective
Answer: B. Contains ingredients of unknown precise composition
Complex media contain peptone, yeast extract, or other ingredients whose exact chemical composition is not fully known. They support the growth of many microorganisms.
Complex media contain peptone, yeast extract, or other ingredients whose exact chemical composition is not fully known. They support the growth of many microorganisms.
Which method is most suitable for obtaining isolated colonies from a mixed culture when the number of viable cells must also be estimated?
- A. Streak plate method
- B. Pour plate method
- C. Slant culture method
- D. Broth culture method
Answer: B. Pour plate method
The pour plate method can be used to count viable cells and also produce isolated colonies. It is useful when both isolation and enumeration are needed.
The pour plate method can be used to count viable cells and also produce isolated colonies. It is useful when both isolation and enumeration are needed.
Which pairing is correctly matched?
- A. Streak plate — estimation of viable count
- B. Pour plate — isolation of colonies and viable count
- C. Slant culture — only anaerobic cultivation
- D. Anaerobic jar — growth of aerobic bacteria with extra oxygen
Answer: B. Pour plate — isolation of colonies and viable count
Pour plate is used for both colony isolation and viable count estimation. The other pairs are incorrectly matched with their main uses.
Pour plate is used for both colony isolation and viable count estimation. The other pairs are incorrectly matched with their main uses.