Chapter 5: Plant Tissue Culture — Online MCQ Test
BOTANY · CLASS 12th · Tamil Nadu State Board
Practice Chapter 5: Plant Tissue Culture with a free chapter-wise online MCQ test.
This chapter covers: Focusing on plant biotechnology this chapter covers totipotency sterilization techniques and culture media preparation. Students explore micropropagation somaclonal variation proto....
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Chapter 5: Plant Tissue Culture — Important Questions & Answers
Who is considered the 'Father of Plant Tissue Culture' for proposing the concept of cellular totipotency in 1902?
- A. F.C. Steward
- B. Gottlieb Haberlandt
- C. E.C. Cocking
- D. Guha and Maheshwari
Answer: B. Gottlieb Haberlandt
Gottlieb Haberlandt proposed the concept of cellular totipotency and was the first to attempt culturing isolated plant cells, earning him the title 'Father of Plant Tissue Culture'.
Gottlieb Haberlandt proposed the concept of cellular totipotency and was the first to attempt culturing isolated plant cells, earning him the title 'Father of Plant Tissue Culture'.
What is the standard temperature and pressure used for autoclaving culture media in a plant tissue culture laboratory?
- A. 100°C and 10 psi for 10 minutes
- B. 121°C and 15 psi for 15 to 30 minutes
- C. 150°C and 20 psi for 30 minutes
- D. 80°C and 5 psi for 60 minutes
Answer: B. 121°C and 15 psi for 15 to 30 minutes
Autoclaving is done at 121°C and 15 pounds per square inch (psi) pressure for 15-30 minutes to sterilize wet culture media and eliminate microbial contamination.
Autoclaving is done at 121°C and 15 pounds per square inch (psi) pressure for 15-30 minutes to sterilize wet culture media and eliminate microbial contamination.
To induce shoot regeneration (caulogenesis) from a callus in vitro, which of the following phytohormone balances is required?
- A. High auxin to low cytokinin ratio
- B. High cytokinin to low auxin ratio
- C. Equal ratio of auxin and gibberellin
- D. High abscisic acid to low gibberellin ratio
Answer: B. High cytokinin to low auxin ratio
Skoog and Miller established that a high cytokinin to auxin ratio promotes shoot regeneration (caulogenesis), whereas a high auxin to cytokinin ratio promotes root regeneration (rhizogenesis).
Skoog and Miller established that a high cytokinin to auxin ratio promotes shoot regeneration (caulogenesis), whereas a high auxin to cytokinin ratio promotes root regeneration (rhizogenesis).
In the history of plant tissue culture, androgenic haploids were first successfully produced by Guha and Maheshwari using which explant?
- A. Ovary of Datura innoxia
- B. Anthers of Datura innoxia
- C. Pollen of Nicotiana tabacum
- D. Endosperm of Zea mays
Answer: B. Anthers of Datura innoxia
In 1964, Guha and Maheshwari reported the direct development of haploid embryoids from the microspores of Datura innoxia by culturing its anthers.
In 1964, Guha and Maheshwari reported the direct development of haploid embryoids from the microspores of Datura innoxia by culturing its anthers.
Shikonin, an important secondary metabolite, is commercially produced from cell suspension cultures of Lithospermum erythrorhizon. Which strategy is specifically applied to release intracellular secondary metabolites into the culture medium without destroying the cells?
- A. Elicitation
- B. Permeabilization
- C. Immobilization
- D. Biotransformation
Answer: B. Permeabilization
Permeabilization involves treating cultured cells with chemicals like DMSO to create temporary pores in the cell membrane, allowing the intracellular secondary metabolites to be harvested without cell destruction.
Permeabilization involves treating cultured cells with chemicals like DMSO to create temporary pores in the cell membrane, allowing the intracellular secondary metabolites to be harvested without cell destruction.