Chapter Exam

Chapter 5: Plant Tissue Culture — Online MCQ Test

Q: Who is considered the 'Father of Plant Tissue Culture' for proposing the concept of cellular totipotency in 1902?

Answer: B. Gottlieb Haberlandt — Gottlieb Haberlandt proposed the concept of cellular totipotency and was the first to attempt culturing isolated plant cells, earning him the title 'Father of Plant Tissue Culture'.

Q: What is the standard temperature and pressure used for autoclaving culture media in a plant tissue culture laboratory?

Answer: B. 121°C and 15 psi for 15 to 30 minutes — Autoclaving is done at 121°C and 15 pounds per square inch (psi) pressure for 15-30 minutes to sterilize wet culture media and eliminate microbial contamination.

Q: To induce shoot regeneration (caulogenesis) from a callus in vitro, which of the following phytohormone balances is required?

Answer: B. High cytokinin to low auxin ratio — Skoog and Miller established that a high cytokinin to auxin ratio promotes shoot regeneration (caulogenesis), whereas a high auxin to cytokinin ratio promotes root regeneration (rhizogenesis).

Q: In the history of plant tissue culture, androgenic haploids were first successfully produced by Guha and Maheshwari using which explant?

Answer: B. Anthers of Datura innoxia — In 1964, Guha and Maheshwari reported the direct development of haploid embryoids from the microspores of Datura innoxia by culturing its anthers.

Q: Shikonin, an important secondary metabolite, is commercially produced from cell suspension cultures of Lithospermum erythrorhizon. Which strategy is specifically applied to release intracellular secondary metabolites into the culture medium without destroying the cells?

Answer: B. Permeabilization — Permeabilization involves treating cultured cells with chemicals like DMSO to create temporary pores in the cell membrane, allowing the intracellular secondary metabolites to be harvested without cell destruction.

BOTANY · CLASS 12th · Tamil Nadu State Board

Practice Chapter 5: Plant Tissue Culture with a free chapter-wise online MCQ test for Tamil Nadu State Board CLASS 12th BOTANY. This chapter covers: Focusing on plant biotechnology this chapter covers totipotency sterilization techniques and culture media preparation. Students explore micropropagation somaclonal variation proto.... AI-generated questions from basic to board-exam level, with instant results and explanations.

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Chapter 5: Plant Tissue Culture — Important Questions & Answers (FAQ)

Frequently asked questions from Tamil Nadu State Board CLASS 12th BOTANY — Chapter 5: Plant Tissue Culture, with answers and explanations. These are sample questions; the exam has its own separate question set.

Who is considered the 'Father of Plant Tissue Culture' for proposing the concept of cellular totipotency in 1902?

A. F.C. Steward
B. Gottlieb Haberlandt ✓
C. E.C. Cocking
D. Guha and Maheshwari

Answer: B. Gottlieb Haberlandt
Gottlieb Haberlandt proposed the concept of cellular totipotency and was the first to attempt culturing isolated plant cells, earning him the title 'Father of Plant Tissue Culture'.

What is the standard temperature and pressure used for autoclaving culture media in a plant tissue culture laboratory?

A. 100°C and 10 psi for 10 minutes
B. 121°C and 15 psi for 15 to 30 minutes ✓
C. 150°C and 20 psi for 30 minutes
D. 80°C and 5 psi for 60 minutes

Answer: B. 121°C and 15 psi for 15 to 30 minutes
Autoclaving is done at 121°C and 15 pounds per square inch (psi) pressure for 15-30 minutes to sterilize wet culture media and eliminate microbial contamination.

To induce shoot regeneration (caulogenesis) from a callus in vitro, which of the following phytohormone balances is required?

A. High auxin to low cytokinin ratio
B. High cytokinin to low auxin ratio ✓
C. Equal ratio of auxin and gibberellin
D. High abscisic acid to low gibberellin ratio

Answer: B. High cytokinin to low auxin ratio
Skoog and Miller established that a high cytokinin to auxin ratio promotes shoot regeneration (caulogenesis), whereas a high auxin to cytokinin ratio promotes root regeneration (rhizogenesis).

In the history of plant tissue culture, androgenic haploids were first successfully produced by Guha and Maheshwari using which explant?

A. Ovary of Datura innoxia
B. Anthers of Datura innoxia ✓
C. Pollen of Nicotiana tabacum
D. Endosperm of Zea mays

Answer: B. Anthers of Datura innoxia
In 1964, Guha and Maheshwari reported the direct development of haploid embryoids from the microspores of Datura innoxia by culturing its anthers.

Shikonin, an important secondary metabolite, is commercially produced from cell suspension cultures of Lithospermum erythrorhizon. Which strategy is specifically applied to release intracellular secondary metabolites into the culture medium without destroying the cells?

A. Elicitation
B. Permeabilization ✓
C. Immobilization
D. Biotransformation

Answer: B. Permeabilization
Permeabilization involves treating cultured cells with chemicals like DMSO to create temporary pores in the cell membrane, allowing the intracellular secondary metabolites to be harvested without cell destruction.

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