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Chapter 10: Applications of Biotechnology — Online MCQ Test

ZOOLOGY · CLASS 12th · Tamil Nadu State Board
Practice Chapter 10: Applications of Biotechnology with a free chapter-wise online MCQ test. This chapter covers: This chapter covers recombinant DNA technology gene therapy transgenic animals insulin production stem cell research and ethical issues in biological engineering.. AI-generated questions from basic to board-exam level, with instant results and explanations.

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Chapter 10: Applications of Biotechnology — Important Questions & Answers

Which enzyme is commonly used to cut DNA at specific sequences in recombinant DNA technology?
  • A. DNA ligase
  • B. Restriction endonuclease
  • C. DNA polymerase
  • D. RNA polymerase
Answer: B. Restriction endonuclease
Restriction endonucleases recognize specific DNA sequences and cut the DNA at or near those sites. They are essential tools in recombinant DNA technology.
Human insulin produced using recombinant DNA technology is popularly known as:
  • A. Somatotropin
  • B. Humulin
  • C. Interferon
  • D. Erythropoietin
Answer: B. Humulin
Humulin is the recombinant human insulin produced by genetic engineering. It is used for treating diabetes mellitus.
In recombinant DNA technology, the function of DNA ligase is to:
  • A. Cut DNA at specific sites
  • B. Join DNA fragments together
  • C. Copy RNA into DNA
  • D. Destroy unwanted proteins
Answer: B. Join DNA fragments together
DNA ligase acts as a molecular glue and joins DNA fragments by forming phosphodiester bonds. It is used after DNA fragments are cut by restriction enzymes.
Which statement best explains why stem cells are considered important in regenerative medicine?
  • A. They can only store nutrients
  • B. They can replace damaged cells and tissues
  • C. They always remain inactive
  • D. They produce antibodies exclusively
Answer: B. They can replace damaged cells and tissues
Stem cells can divide and differentiate into specialized cells, making them useful for repairing or replacing damaged tissues. This is the basis of regenerative medicine.
Which option correctly matches the biotechnology product with its application?
  • A. Humulin — production of human insulin
  • B. Rosie — production of vaccine
  • C. Stem cells — pesticide development
  • D. Restriction enzyme — tissue culture medium
Answer: A. Humulin — production of human insulin
Humulin is recombinant human insulin used in the treatment of diabetes. The other pairs are mismatched and do not represent correct applications.