Chapter Exam

Chapter 10: Applications of Biotechnology — Online MCQ Test

Q: Which enzyme is commonly used to cut DNA at specific sequences in recombinant DNA technology?

Answer: B. Restriction endonuclease — Restriction endonucleases recognize specific DNA sequences and cut the DNA at or near those sites. They are essential tools in recombinant DNA technology.

Q: Human insulin produced using recombinant DNA technology is popularly known as:

Answer: B. Humulin — Humulin is the recombinant human insulin produced by genetic engineering. It is used for treating diabetes mellitus.

Q: In recombinant DNA technology, the function of DNA ligase is to:

Answer: B. Join DNA fragments together — DNA ligase acts as a molecular glue and joins DNA fragments by forming phosphodiester bonds. It is used after DNA fragments are cut by restriction enzymes.

Q: Which statement best explains why stem cells are considered important in regenerative medicine?

Answer: B. They can replace damaged cells and tissues — Stem cells can divide and differentiate into specialized cells, making them useful for repairing or replacing damaged tissues. This is the basis of regenerative medicine.

Q: Which option correctly matches the biotechnology product with its application?

Answer: A. Humulin — production of human insulin — Humulin is recombinant human insulin used in the treatment of diabetes. The other pairs are mismatched and do not represent correct applications.

ZOOLOGY · CLASS 12th · Tamil Nadu State Board

Practice Chapter 10: Applications of Biotechnology with a free chapter-wise online MCQ test for Tamil Nadu State Board CLASS 12th ZOOLOGY. This chapter covers: This chapter covers recombinant DNA technology gene therapy transgenic animals insulin production stem cell research and ethical issues in biological engineering.. AI-generated questions from basic to board-exam level, with instant results and explanations.

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Chapter 10: Applications of Biotechnology — Important Questions & Answers (FAQ)

Frequently asked questions from Tamil Nadu State Board CLASS 12th ZOOLOGY — Chapter 10: Applications of Biotechnology, with answers and explanations. These are sample questions; the exam has its own separate question set.

Which enzyme is commonly used to cut DNA at specific sequences in recombinant DNA technology?

A. DNA ligase
B. Restriction endonuclease ✓
C. DNA polymerase
D. RNA polymerase

Answer: B. Restriction endonuclease
Restriction endonucleases recognize specific DNA sequences and cut the DNA at or near those sites. They are essential tools in recombinant DNA technology.

Human insulin produced using recombinant DNA technology is popularly known as:

A. Somatotropin
B. Humulin ✓
C. Interferon
D. Erythropoietin

Answer: B. Humulin
Humulin is the recombinant human insulin produced by genetic engineering. It is used for treating diabetes mellitus.

In recombinant DNA technology, the function of DNA ligase is to:

A. Cut DNA at specific sites
B. Join DNA fragments together ✓
C. Copy RNA into DNA
D. Destroy unwanted proteins

Answer: B. Join DNA fragments together
DNA ligase acts as a molecular glue and joins DNA fragments by forming phosphodiester bonds. It is used after DNA fragments are cut by restriction enzymes.

Which statement best explains why stem cells are considered important in regenerative medicine?

A. They can only store nutrients
B. They can replace damaged cells and tissues ✓
C. They always remain inactive
D. They produce antibodies exclusively

Answer: B. They can replace damaged cells and tissues
Stem cells can divide and differentiate into specialized cells, making them useful for repairing or replacing damaged tissues. This is the basis of regenerative medicine.

Which option correctly matches the biotechnology product with its application?

A. Humulin — production of human insulin ✓
B. Rosie — production of vaccine
C. Stem cells — pesticide development
D. Restriction enzyme — tissue culture medium

Answer: A. Humulin — production of human insulin
Humulin is recombinant human insulin used in the treatment of diabetes. The other pairs are mismatched and do not represent correct applications.

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