Chapter Exam

Chapter 8: Animal Cell Culture — Online MCQ Test

Q: A culture started directly from cells, tissues, or organs taken from an animal is called:

Answer: A. Primary culture — Primary culture is established directly from animal tissue and contains cells that have not yet been repeatedly subcultured.

BIOTECHNOLOGY · Grade 12 · CBSE(NCERT)

Practice Chapter 8: Animal Cell Culture with a free chapter-wise online MCQ test for CBSE(NCERT) Grade 12 BIOTECHNOLOGY. This chapter covers: Animal Cell Culture Primary Culture Cell Line In Vitro Culture Growth Medium Monoclonal Antibodies Vaccine Production Cell Maintenance Tissue Culture. AI-generated questions from basic to board-exam level, with instant results and explanations.

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Chapter 8: Animal Cell Culture — Important Questions & Answers (FAQ)

Frequently asked questions from CBSE(NCERT) Grade 12 BIOTECHNOLOGY — Chapter 8: Animal Cell Culture, with answers and explanations. These are sample questions; the exam has its own separate question set.

Animal cell culture is best described as the growth of animal cells:

A. Outside the organism under controlled conditions ✓
B. Only inside a living animal
C. Only in soil-based nutrient medium
D. Without any nutrient requirement

Answer: A. Outside the organism under controlled conditions
Animal cell culture involves maintaining and growing animal cells in vitro, i.e., outside the body under controlled laboratory conditions.

A culture started directly from cells, tissues, or organs taken from an animal is called:

A. Primary culture ✓
B. Continuous cell line
C. Hybridoma culture
D. Cloned culture

Answer: A. Primary culture
Primary culture is established directly from animal tissue and contains cells that have not yet been repeatedly subcultured.

Which sequence is most appropriate for establishing a primary animal cell culture from a tissue sample?

A. Collect tissue aseptically → Disaggregate cells → Seed in suitable medium ✓
B. Freeze tissue → Add agar → Expose to open air
C. Boil tissue → Add antibiotics → Incubate without medium
D. Dry tissue → Grind with soil → Store at room temperature

Answer: A. Collect tissue aseptically → Disaggregate cells → Seed in suitable medium
Primary culture requires aseptic collection, mechanical or enzymatic disaggregation, and inoculation into an appropriate culture medium.

A researcher has cells from a freshly isolated tissue that have not yet been passaged. After several subcultures, they are referred to as a cell line. Which statement is correct?

A. The initial culture is primary culture; repeated subculturing can give rise to a cell line ✓
B. The initial culture is a continuous cell line; subculture makes it primary
C. A primary culture and a cell line are always identical terms
D. Subculturing always kills all animal cells

Answer: A. The initial culture is primary culture; repeated subculturing can give rise to a cell line
Primary culture is derived directly from tissue, while a cell line is obtained after the cells are subcultured or passaged.

In HAT selection during hybridoma production, which combination allows hybridoma cells to survive?

A. Immortality from myeloma cells and salvage pathway enzyme from B cells ✓
B. Antibody production from myeloma cells and absence of nuclei from B cells
C. Cell wall formation from B cells and plasmids from myeloma cells
D. Agar digestion from myeloma cells and chlorophyll from B cells

Answer: A. Immortality from myeloma cells and salvage pathway enzyme from B cells
In HAT medium, aminopterin blocks de novo nucleotide synthesis; hybridomas survive because they inherit immortality from myeloma cells and salvage pathway ability from B cells.