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CBSE(NCERT) · Grade 12 · Biotechnology

CBSE(NCERT) GRADE 12 BIOTECHNOLOGY 2022 SET1

13 questions from this Grade 12 Biotechnology paper. Log in as a Grade 12 student to view solutions.

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Q7 short answer 3 marks
(a) Why is regulation of pH important for survival of mammalian cells in culture? Write two reasons. (b) How does bicarbonate $CO_2$ buffering system work?

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Q8 short answer 3 marks
What is RefSeq? Give its significance. (any 2 points)

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Reading Passage

Continuous Culture : To get a continuous supply of microbial growth, continuous culture is used. The growth medium is designed in such a way that one of the nutrients is in limited quantity. Nutrients are continually added to the bioreactor and spent culture broth is removed at the same time. This culture helps to obtain stable product quality and higher productivity per unit volume, so this technique is widely used for production of both microbial biomass as well as their metabolites.

Q10 short answer 2 marks
Continuous Culture : To get a continuous supply of microbial growth, continuous culture is used. The growth medium is designed in such a way that one of the nutrients is in limited quantity. Nutrients are continually added to the bioreactor and spent culture broth is removed at the same time. This culture helps to obtain stable product quality and higher productivity per unit volume, so this technique is widely used for production of both microbial biomass as well as their metabolites. (i) Differentiate between turbidostat and chemostat.

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Q11 short answer 3 marks
How can a farmer eliminate the process of emasculation to prevent unnecessary pollination in his maize crop? Can normal pollen formation be ensured at the onset of breeding seasons?

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Q11 short answer 1 mark
Which type of microbial culture is shown in this graph ?

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Q12 short answer 3 marks
Draw a labelled diagram of an artificial seed. Why are these seeds produced?

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Q12 short answer 3 marks
What are artificial seeds? Why are they produced?

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Q12 short answer 1 mark
What is Batch Culture ?

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Reading Passage

Microbial cultures have immense potential for production of very useful compounds. These cultures can be exploited mainly to produce different metabolites which include enzymes, steroids, antibiotics, therapeutic proteins, microbial leaching, biodegradable waste treatment, etc.

Q13 short answer 1 mark
(ii) Define DSP. Why is it advisable to use less number of steps in a DSP protocol?

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Q13 short answer 1 mark
(iii) What are excipients?

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Q13 short answer 1 mark
(iv) Which organisms are referred to as GRAS?

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Q13 short answer 1 mark
(v) Briefly mention two biosafety issues specific in microbial technology.

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Reading Passage

Continuous Culture : To get a continuous supply of microbial growth, continuous culture is used. The growth medium is designed in such a way that one of the nutrients is in limited quantity. Nutrients are continually added to the bioreactor and spent culture broth is removed at the same time. This culture helps to obtain stable product quality and higher productivity per unit volume, so this technique is widely used for production of both microbial biomass as well as their metabolites.

Q13 short answer
How is continuous culture different from Fed-Batch culture in terms of volume in the culture vessel ? In which type of method can microbial culture be maintained in an extended log or stationary phase ?

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