Q3
mcq
1 mark
Which of the following statement is not correct about DNA sequencing by Sanger Method ?
- A. Primers can only be extended using the single strand DNA as template.
- B. Four ddNTPs ddATP, ddUTP, ddCTP and ddGTP are needed.
- C. DNA polymerase enzyme is needed.
- D. ddNTPs are added to terminate chain (DNA) extending.
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Q4
mcq
1 mark
Charge relay system operating in chymotrypsin enzyme consists of following amino acids at positions 57, 102 and 195 respectively :
- A. his-asp-ser
- B. his-lys-ser
- C. his-lys-asp
- D. lys-his-ser
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Q5
mcq
1 mark
Number of predicted genes is 6340 and 70% part of the genome codes for protein in :
- A. Yeast
- B. Caenorhabditis worm
- C. Arabidopsis weed
- D. Bacterium E. coli
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Q6
mcq
1 mark
SCID disease is caused by absence of enzyme :
- A. Adenosine synthase
- B. Adenosine deaminase
- C. Adenosine phosphatase
- D. Adenosine aminase
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Q7
mcq
1 mark
Technique of introducing colours into DNA by Nick Translation, was developed by :
- A. Rigby and Paul Berg
- B. Rous and Jones
- C. Kary Mullis and Paul Berg
- D. Edward Sanger
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Q8
mcq
1 mark
Alcaligenes eutrophus is associated with the production of :
- A. Streptomycin
- B. Penicillin
- C. Hepatitis antigen
- D. Poly 3-hydroxybutyrate
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Q9
mcq
1 mark
Expression vector has which of the following properties ?
- A. High copy number and stable
- B. Easy to transfer in eukaryote cells
- C. Maximise production of foreign protein
- D. Both (a) and (c)
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Q10
mcq
1 mark
Artemisin obtained from Artemisia species is used as
- A. Antimalarial
- B. Antifertility
- C. Anticarcinogenic
- D. Analgesic
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Q11
mcq
1 mark
Transgenic plants which over-express compounds like sugars, mannitol and proline
- A. are pest resistant
- B. are abiotic stress tolerant
- C. produce more secondary metabolites as drugs and other important metabolites
- D. are weedicide tolerant
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Q12
mcq
1 mark
Person who lacks the ability to produce Factor VIII has heritable genetic disorder :
- A. Haemophilia A
- B. Haemophilia B
- C. Christmas disease
- D. Stroke
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Q13
mcq
1 mark
Assertion (A): Protoplasts are plant cells without cell wall.
Reason (R): Protoplasts can be utilized for producing somatic hybrids.
- A. Both Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of Assertion (A).
- B. Both Assertion (A) and Reason (R) are true, but Reason (R) is not the correct explanation of Assertion (A).
- C. Assertion (A) is true, but Reason (R) is false.
- D. Assertion (A) is false, but Reason (R) is true.
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Q14
mcq
1 mark
Assertion (A): Homology is defined as similarity due to common ancestry.
Reason (R): DNA sequences obtained from different individuals of same species show homology due to similarity in DNA sequence.
- A. Both Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of Assertion (A).
- B. Both Assertion (A) and Reason (R) are true, but Reason (R) is not the correct explanation of Assertion (A).
- C. Assertion (A) is true, but Reason (R) is false.
- D. Assertion (A) is false, but Reason (R) is true.
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Q15
mcq
1 mark
Assertion (A): Insert and vector DNA are cut with the help of Restriction endonuclease.
Reason (R): Ligase can produce the nick in the recombinant DNA molecule.
- A. Both Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of Assertion (A).
- B. Both Assertion (A) and Reason (R) are true, but Reason (R) is not the correct explanation of Assertion (A).
- C. Assertion (A) is true, but Reason (R) is false.
- D. Assertion (A) is false, but Reason (R) is true.
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Q16
mcq
1 mark
Assertion (A): For the expression of eukaryotic proteins, E. coli is preferred.
Reason (R): E. coli is easy to grow and manipulate, so is used to express eukaryotic proteins.
- A. Both Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of Assertion (A).
- B. Both Assertion (A) and Reason (R) are true, but Reason (R) is not the correct explanation of Assertion (A).
- C. Assertion (A) is true, but Reason (R) is false.
- D. Assertion (A) is false, but Reason (R) is true.
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Q17
short answer
2 marks
(a) For selection of recombinants having lacZ gene on vector DNA, it was plated on medium containing sucrose with a chromogen dye. (Show sucrose breakdown) No result was obtained. Why?
(b) What can be done to select these recombinants?
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Q18
short answer
2 marks
How does mosquito repellant work? Mention its major chemical constituents.
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Q19
short answer
2 marks
6 million to 32 million sites in the human genome, where SNPs can occur, but still they may or may not affect gene function. Justify the statement giving reasons.
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Q20
short answer
2 marks
(a) Differentiate between Primary cell cultures and Secondary cell cultures.
OR
(b) Differentiate between Finite cell lines and Continuous cell lines.
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Q21
short answer
2 marks
How will you perform laboratory screening for micro-organism producing an antibiotic?
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Q22
short answer
3 marks
Give reason for the following:
(a) Baffle flasks have V-shaped notch.
(b) Excess foaming in microbiological processes is a problem.
(c) Slaughter-house wastes are used to prepare media for microbiological processes.
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Q23
short answer
3 marks
(a) How are mouse models created? Write two major applications in the field of stem cell technology.
OR
(b) Complete the flowchart for creating gene knockout by fill
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Q24
short answer
3 marks
Pichia pastoris is the most suitable host to express eukaryotic gene. Support the statement giving three reasons.
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Q25
short answer
3 marks
Transgenic crops are facing challenges globally. What are the major concerns about these GM crops ?
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Q27
short answer
3 marks
How is MALDI (Matrix Assisted Laser Desorption Ionisation) used to volatalise and protonate peptides and proteins ?
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Q28
short answer
3 marks
(a) What are the essential features that a vector should possess ?
(b) What are shuttle vectors ?
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Q28
short answer
2 marks
SNPs can decide our susceptibility to, or protection from, all kinds of diseases. Give any one example to support the same.
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Single nucleotide polymorphisms (SNPs) are common genetic variations among people. Each SNP represents a difference in a SNPs can help scientists to locate genes associated with disease. Research is going on to identify SNPs associated with diseases like diabetes, cancer etc.
Q29
long answer
(a) Read the passage and answer the question that follows :
Single nucleotide polymorphisms (SNPs) are common genetic variations among people. Each SNP represents a difference in a SNPs can help scientists to locate genes associated with disease. Research is going on to identify SNPs associated with diseases like diabetes, cancer etc.
(i) How can SNPs help physicians ?
(ii) SNPs can decide our susceptibility to, or protection from all kinds of diseases. Give any one example to support the same.
(iii) Why is SNP analysis used in population genetics ?
OR
(iii) Do SNPs occur only in coding regions of human genome or in both coding and non-coding regions ?
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Q29
short answer
1 mark
Why is SNP analysis used in population genetics?
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Q30
short answer
1 mark
Do SNPs occur only in coding regions of the human genome or in both coding and non-coding regions?
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Q30
short answer
1 mark
Animal cell culture. Animal cells can be grown in glass or plastic vessels with nutrient medium. These need to be given fresh medium periodically. The infant animal cells grow only to adulthood and not any further. While growing, they show phenomenon of contact inhibition, but
(i) What is contact inhibition?
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Q31
long answer
5 marks
(a) (i) Differentiate between primary and secondary metabolites.
(ii) Name three industrially important plant secondary metabolites produced through cell and tissue culture, along with their use.
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Q31
short answer
5 marks
(b) (i) According to WHO estimates, approximately 228 million children are affected due to Vitamin A deficiency. How has genetic engineering provided/developed a solution to the same? Explain.
(ii) Why is nutritional quality of cereals and legumes limited?
(iii) Name two essential amino acids which are deficient in pulses.
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Q32
short answer
1 mark
Which property of cells is studied by oncologists to determine whether cells are normal or cancerous?
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Q32
long answer
4 marks
(a) (i) Draw diagram showing schematic representation of the basic steps in Recombinant DNA Technology.
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Q32
short answer
3 marks
(b) (i) Describe the technique to detect microbes from environmental samples present in very small numbers.
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Q33
short answer
2 marks
As in vivo environment is different from in vitro environment, how does it affect cells grown in culture? Mention any two.
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Q33
long answer
5 marks
(a) (i) How are conventional vaccines different from recombinant vaccines?
(ii) What are epitopes?
(iii) What are the advantages of preparing vaccines using epitopes?
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Q33
short answer
(b) (i) Branched Chain Amino acids (BCAA) are essential for the biosynthesis of muscle protein. How? Explain.
(ii) What is Biological value of prot
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Q34
short answer
2 marks
Mortality is associated with all normal animal cells. Justify giving reason.
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Q38
short answer
4 marks
(For visually-Impaired candidates only in lieu of Q. 32 (a) (i)) Write all the four defined steps of Recombinant DNA Technology in correct sequence. Name any two scientists who were involved in generation of first Recombinant DNA molecule. Name two products produced commercially using this technology.
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Q39
short answer
1 mark
Why are Type II restriction enzymes preferred in the above mentioned technology?
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Q41
short answer
2 marks
Why is this technique getting popular in diagnostics?
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Q44
long answer
2 marks
How are conventional vaccines different from recombinant vaccines?
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Q46
long answer
2 marks
What are the advantages of preparing vaccines using epitopes?
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Q47
long answer
3 marks
Branched Chain Amino acids (BCAA) are essential for the biosynthesis of muscle protein. How? Explain.
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Q48
short answer
1 mark
What is Biological value of proteins?
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Q49
long answer
1 mark
What is Protein Efficiency Ratio ? Arrange the following proteins in decreasing order of protein efficiency ratio : Milk, Casein, Soya, Wheat, Whey
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