Chapter Exam

Chapter 9: Biotechnology Principles and Processes — Online MCQ Test

Q: Which enzyme is used to join two DNA fragments by forming phosphodiester bonds?

Answer: B. DNA ligase — DNA ligase joins DNA fragments by sealing nicks and forming phosphodiester bonds between adjacent nucleotides.

Q: Restriction enzymes are also known as:

Answer: A. Molecular scissors — Restriction enzymes cut DNA at specific recognition sequences, so they are commonly called molecular scissors.

Q: Which of the following is NOT an essential feature of a cloning vector according to NCERT?

Answer: D. Centromere for mitosis in bacteria — Bacterial cloning vectors require ori, selectable marker and cloning sites; bacteria do not use centromeres for mitosis.

Q: In the naming of EcoRI, what does the letter R represent?

Answer: A. The strain of Escherichia coli — In EcoRI, E represents Escherichia, co represents coli, R represents the strain, and I indicates it was the first enzyme isolated from that strain.

BIOLOGY · CLASS 12 SECOND PUC · Karnataka State Board

Practice Chapter 9: Biotechnology Principles and Processes with a free chapter-wise online MCQ test for Karnataka State Board CLASS 12 SECOND PUC BIOLOGY. This chapter covers: Biotechnology Recombinant DNA Restriction Enzymes DNA Ligase Cloning Vector Gene Cloning PCR Bioreactor Genetic Engineering Transformation. AI-generated questions from basic to board-exam level, with instant results and explanations.

Questions

20m

Time Limit

Attempts Left

10 random questions from this chapter (mixed difficulty)
Questions you've seen before won't repeat until the pool resets
You have 20 minutes — exam auto-submits when time is up
Maximum 3 attempts per chapter
Results and explanations shown immediately after submission

New here? Register free — includes 3 free chapter exams.

Chapter 9: Biotechnology Principles and Processes — Important Questions & Answers (FAQ)

Frequently asked questions from Karnataka State Board CLASS 12 SECOND PUC BIOLOGY — Chapter 9: Biotechnology Principles and Processes, with answers and explanations. These are sample questions; the exam has its own separate question set.

Which enzyme is used to join two DNA fragments by forming phosphodiester bonds?

A. DNA polymerase
B. DNA ligase ✓
C. Restriction endonuclease
D. RNA polymerase

Answer: B. DNA ligase
DNA ligase joins DNA fragments by sealing nicks and forming phosphodiester bonds between adjacent nucleotides.

Restriction enzymes are also known as:

A. Molecular scissors ✓
B. Molecular glue
C. Cloning vehicles
D. Thermal cyclers

Answer: A. Molecular scissors
Restriction enzymes cut DNA at specific recognition sequences, so they are commonly called molecular scissors.

Which of the following is NOT an essential feature of a cloning vector according to NCERT?

A. Origin of replication
B. Selectable marker
C. Cloning site
D. Centromere for mitosis in bacteria ✓

Answer: D. Centromere for mitosis in bacteria
Bacterial cloning vectors require ori, selectable marker and cloning sites; bacteria do not use centromeres for mitosis.

EcoRI recognizes the sequence 5'-GAATTC-3'. After cutting, it produces sticky ends because:

A. It cuts both strands exactly at the centre, producing blunt ends
B. It cuts the two DNA strands at different positions, producing overhangs ✓
C. It removes all adenine bases from DNA
D. It converts double-stranded DNA into RNA

Answer: B. It cuts the two DNA strands at different positions, producing overhangs
EcoRI cuts asymmetrically between G and A on opposite strands, producing single-stranded overhangs called sticky ends.

In the naming of EcoRI, what does the letter R represent?

A. The strain of Escherichia coli ✓
B. The genus of the source organism
C. The species of the source organism
D. The order in which the enzyme was isolated

Answer: A. The strain of Escherichia coli
In EcoRI, E represents Escherichia, co represents coli, R represents the strain, and I indicates it was the first enzyme isolated from that strain.