Chapter Exam

Unit 5 Chapter 11: Biotechnology Principles and Processes — Online MCQ Test

Q: Which enzyme cuts DNA at specific recognition sites in recombinant DNA technology?

Answer: B. Restriction endonuclease — Restriction endonucleases recognize specific DNA sequences and cleave the DNA at or near these sites. They are essential for making recombinant DNA.

Q: A plasmid used to transfer foreign DNA into a host cell is called a ____.

Answer: A. vector — A vector is a DNA molecule that carries foreign DNA into a host cell and allows its replication. Plasmids are commonly used vectors in biotechnology.

Q: Which statement best explains why restriction enzymes are called molecular scissors?

Answer: B. They cut DNA at specific palindromic sequences — Restriction enzymes cut DNA at specific recognition sequences, often palindromic in nature. Because of this cutting action, they are called molecular scissors.

BOTANY · CLASS 12 INTERMEDIATE 2 YEAR · Telangana State Board

Practice Unit 5 Chapter 11: Biotechnology Principles and Processes with a free chapter-wise online MCQ test for Telangana State Board CLASS 12 INTERMEDIATE 2 YEAR BOTANY. This chapter covers: This chapter covers recombinant DNA technology principles restriction enzymes vectors competent host transformations PCR technology downstream processing and bioprocess engineering.... AI-generated questions from basic to board-exam level, with instant results and explanations.

Questions

20m

Time Limit

Attempts Left

10 random questions from this chapter (mixed difficulty)
Questions you've seen before won't repeat until the pool resets
You have 20 minutes — exam auto-submits when time is up
Maximum 3 attempts per chapter
Results and explanations shown immediately after submission

New here? Register free — includes 3 free chapter exams.

Unit 5 Chapter 11: Biotechnology Principles and Processes — Important Questions & Answers (FAQ)

Frequently asked questions from Telangana State Board CLASS 12 INTERMEDIATE 2 YEAR BOTANY — Unit 5 Chapter 11: Biotechnology Principles and Processes, with answers and explanations. These are sample questions; the exam has its own separate question set.

Which enzyme cuts DNA at specific recognition sites in recombinant DNA technology?

A. DNA ligase
B. Restriction endonuclease ✓
C. DNA polymerase
D. RNA polymerase

Answer: B. Restriction endonuclease
Restriction endonucleases recognize specific DNA sequences and cleave the DNA at or near these sites. They are essential for making recombinant DNA.

A plasmid used to transfer foreign DNA into a host cell is called a ____.

A. vector ✓
B. probe
C. promoter
D. replicon

Answer: A. vector
A vector is a DNA molecule that carries foreign DNA into a host cell and allows its replication. Plasmids are commonly used vectors in biotechnology.

Why is calcium chloride treatment often used before transformation of bacteria?

A. It digests the bacterial cell wall completely
B. It makes bacterial cells competent to take up DNA ✓
C. It prevents plasmid replication
D. It cuts chromosomal DNA into fragments

Answer: B. It makes bacterial cells competent to take up DNA
Calcium chloride treatment increases the competency of bacterial cells, making them more capable of taking up foreign DNA. This is a standard step before transformation.

Which statement best explains why restriction enzymes are called molecular scissors?

A. They synthesize new DNA strands
B. They cut DNA at specific palindromic sequences ✓
C. They join DNA fragments together
D. They bind only to RNA

Answer: B. They cut DNA at specific palindromic sequences
Restriction enzymes cut DNA at specific recognition sequences, often palindromic in nature. Because of this cutting action, they are called molecular scissors.

Which sequence correctly represents the main order of events in recombinant DNA technology?

A. Isolation of DNA → cutting with restriction enzyme → ligation into vector → transformation into host ✓
B. Transformation into host → ligation into vector → cutting with restriction enzyme → isolation of DNA
C. Ligation into vector → isolation of DNA → transformation into host → cutting with restriction enzyme
D. PCR → downstream processing → restriction digestion → cloning

Answer: A. Isolation of DNA → cutting with restriction enzyme → ligation into vector → transformation into host
Recombinant DNA technology begins with DNA isolation, followed by cutting with restriction enzymes, ligation into a vector, and introduction into a host cell. This is the standard workflow.